Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 30% increase in activity | Agarivorans albus | |
dithiothreitol | 30% increase in activity | Agarivorans albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | strong | Agarivorans albus | |
Cu2+ | strong | Agarivorans albus | |
Fe3+ | strong | Agarivorans albus | |
Mn2+ | strong | Agarivorans albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ratio of Km/kcat value for hydrolysis of neoagarohexaose and neoagarotetraose is 4040 and 810 per s and M, respectively | Agarivorans albus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Agarivorans albus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions required for activity | Agarivorans albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Agarivorans albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agarivorans albus | - |
isolated from gut of a turban shell | - |
Agarivorans albus YKW-34 | - |
isolated from gut of a turban shell | - |
Purification (Comment) | Organism |
---|---|
from culture supernatant | Agarivorans albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Agarivorans albus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.5 | - |
40°C, pH 8.0 | Agarivorans albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | - |
Agarivorans albus | neoagarobiose + neoagarotetraose | about 75% neoagarobiose plus 25% neoagarotetraose | ? | |
agarose + H2O | - |
Agarivorans albus YKW-34 | neoagarobiose + neoagarotetraose | about 75% neoagarobiose plus 25% neoagarotetraose | ? | |
additional information | no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan | Agarivorans albus | ? | - |
? | |
additional information | no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan | Agarivorans albus YKW-34 | ? | - |
? | |
neoagarohexaose + H2O | - |
Agarivorans albus | neoagarobiose | - |
? | |
neoagarohexaose + H2O | - |
Agarivorans albus YKW-34 | neoagarobiose | - |
? | |
neoagarotetraose + H2O | - |
Agarivorans albus | neoagarobiose | - |
? | |
neoagarotetraose + H2O | - |
Agarivorans albus YKW-34 | neoagarobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Agarivorans albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Agarivorans albus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
90% of maximum activity | Agarivorans albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, 95% residual activity. Inactivation above 50°C | Agarivorans albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Agarivorans albus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 80% of maximum activity | Agarivorans albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Agarivorans albus |